阿尔兹海默症的重要致病蛋白 Amyloid-β在体外多个细胞模型中被发现能促进神经细胞衰老和衰老相关的DNA损伤应激。SIRT1长寿蛋白在这个过程中被发现显著下调。通过基因过表达和化合物干预的方式提高SIRT1水平被发现能显著缓解神经细胞衰老

在神经细胞SK-N-SH和SH-SY5Y细胞上通过在不同的时间点上用Amyloid-β处理，免疫荧光检测DNA损伤标记物H2AX的磷酸化水平。同时利用SIRT1抑制剂检测能否阻止阿司匹林对Amyloid-β诱导的神经细胞衰老标志物的缓解作用

SK-N-SH and SH-SY5Y neural cells were treated with Amyloid-β at various time points, and immunofluorescence assay was conducted to detect the phosphorylation level of H2AX, a DNA damage biomarker. Meanwhile, SIRT1 inhibitors were employed to examine whether they could block the alleviating effect of aspirin on Amyloid-β-induced neural cell senescence biomarkers.