Functional omics analyses reveal only minor effects of microRNAs on human somatic stem cell differentiation [mRNA-Seq]

Functional analysis of transcriptome (mRNA and microRNA) and proteome formation during treatment of cord-blood derived stem cell lines (USSC) with retinoic acid-containing XXL-medium to estimate the impact of microRNAs on final proteome. Overall design: NGS analysis (mRNA and microRNAs) and proteome of native and XXL-treated USSC. Bioinformatic target prediction analysis of regulated microRNAs in comparison to regulated proteome. Transfection of USSC with regulated microRNAs, followed by XXL treatment and subsequent proteome anlysis to estimate the contribution of microRNAs to proteome shaping in USSC upon XXL-treatment.