Freshwater Reservoir Amadorio Metagenome

he study aims to describe the microbial community of a freshwater reservoir using high throughput sequencing. The reservoir is called Amadorio and is located in the region of Alicante, Spain. Water was collected and filtered through a series of polycarbonate filters, a 20 micron prefilter, 5 micron (for large cells and eukaryotes) and 0.22 or 0.1 micron (for planktonic microbes). The data deposited in this bioproject originates from two independent samplings. For one sample, DNA was extracted from the 0.1 micron filter (small, planktonic fraction) and 5 micron (large, particulate fraction) filters to obtain genomic DNA for two different size fractions of the microbial community. Both these samples were sequenced by a shotgun metagenomic approach. For the second sample, DNA from the 0.22 micron was cloned into a fosmid library. Approximately 400 fosmids were pooled in a single batch and a total of 14 batches (total ca. 5500 fosmids) were sequenced. All sequencing was done using Illumina HiSeq 2000. Metagenomic reads from each batch are available within this bioproject.