Gene expression profile at the singe-cell level on human induced pluripotent stem cell (hiPSC) derived midbrain dopaminergic neurons

We optimised a new differentiation paradigm to produce midbrain dopaminergic neurons from hiPSCs. To characterise their identity, we performed scRNA-seq to investigate their gene expression and molecular identity. Overall design: hiPSCs were differentiated using small molecule combination for 20 days to produce midbrain dopaminergic (mDA) precursor cells. These were then terminally differentiated into mDA neurons and grown for a further 28 days. The mDA monolayer was then dissociated into a single-cell suspension to perform scRNA-seq.