Sus scrofa domesticus strain:BX-cross Transcriptome or Gene expression

The aim of this study was characterize the transcriptomes of two phentypically distinct cell types in the pig placenta that occur along the fetal maternal interface at day 85 of gestation using a combination of laser capture microdissection and RNA-seq.. As the placenta of the pig is a non-invasive form of placentation, cells along the interface may play a critical role in the formation of microscopic folds that increase the surface area of the placenta. The depth of these folds are also inversely related to the body size of the fetal piglet with small piglets having larger folds than large piglets. Small piglets have a higher rate of mortality and do not gain weight in an efficient manner post-weaning. Determining the function of the cells and what makes them different may shed light on the mechanisms controlling development of the folds and generate new hypothesis's for the development of interventions designed to increase piglet birth-weight, survivability and post-weaning weight gain.

本研究旨在通过结合激光捕获显微切割技术与RNA测序技术，对妊娠85天时位于猪胎盘胎儿母体界面的两种表型不同的细胞类型的转录组进行表征。鉴于猪胎盘是一种非侵入性的胎盘形成方式，界面处的细胞可能在形成增加胎盘表面积的微观褶皱过程中发挥关键作用。这些褶皱的深度与胎儿猪的身体大小呈负相关，体型较小的猪崽具有比体型较大的猪崽更大的褶皱。体型较小的猪崽具有较高的死亡率，并且在断奶后不能有效地增重。确定这些细胞的功能及其差异可能有助于揭示控制褶皱发育的机制，并为旨在提高猪崽出生体重、存活率和断奶后增重的干预措施的开发提供新的假设。