Effect of cadmium on enzyme activities of the acetoclastic pathway upper part from Methanosarcina acetivorans.

All activities were determined by using freshly prepared cytosolic fraction as described in the Methods section. Values are the mean ± SD of at least three independent preparations.Control activities were for AK: 0.75±0.21 U/mg protein (n = 4); for Pta: 1.48±0.8 U/mg protein (n = 4); for CODH/AcCoA synthase with acetyl-CoA: 0.37 U±0.12 U/mg protein (n = 5); and with CO: 0.68±0.11 U/mg protein (n = 3); CA: 26±12 U/mg protein.*Pvs control for independent samples;**Pvs control for paired samples. ND: Not determined.