The Mutational Concordance of Fixed Formalin Paraffin Embedded and Fresh Frozen Gastro-oesophageal Tumours Using Whole Exome Sequencing

This is a feasibility study to assess DNA extracted from formalin-fixed paraffin-embedded (FFPE) gastro-oesophageal tumour for massively parallel sequencing, where we analysed whole exome sequencing data to define the mutational concordance of 16 matched fresh frozen and FFPE gastro-oesophageal tumours (N=32). We assessed DNA integrity prior to sequencing and identified coding mutations in genes that have also been implicated in other cancers.