Migratory and anti-fibrotic programmes define the regenerative potential of human cardiac progenitors

In the present study, scRNAseq was carried out to establish a molecular roadmap for HVP specification and maturation during heart repair as well as HVP properties in response to acute cardiac injury. Transcriptional profiling by scRNAseq was performed at various timepoints during Human ventricular progenitor-differentiation at D0 and D3 towards cardiomyocytes at D21 that were co-cultured on chimeric human- NHP heart slices. scRNAseq was also performed for HVPs and NHP cells at 24h and 48 h after RFA- induced injury. Chimeric heart slices were dissociated and single-cell RNA was isolated from eGFP+ cells at D0, D3 and D21 without RFA, and from eGFP+ and eGFP- cells 24h and 48h after RFA- injury and subsequently subjected to scRNA sequencing.