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Microsatellite exploration in the climbing hydrangea (Hydrangea petiolaris Siebold & Zucc.) transcriptome: A resource for population genetics and functional genomics

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DataONE2024-01-22 更新2025-08-02 收录
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Background Hydrangea petiolaris Siebold & Zucc., also known as climbing hydrangea, is a vine native to the woodlands of Korea, Japan, and Sakhalin Island. It is an economically important ornamental plant with fertile and sterile flowers. Despite the recent increase in Hydrangea breeding and interest in germplasm conservation, relatively little is known about the relationships between Hydrangea species. Results We employed Illumina NovaSeq 6000 sequencing technology to generate a total of 39,945,480 reads, which were assembled into 137,715 contigs. A total of 109,092 filtered transcripts were used to identify microsatellites, and 54,587 microsatellite repeat motifs were revealed within 33,556 contigs. Among these, 4,510 transcripts harboring microsatellites had Gene Ontology annotations, and numerous microsatellite-containing transcripts exhibited associations with genes, including those encoding PPR proteins, aldehyde dehydrogenases, and bHLH transcription factors, related to the re..., , , # Microsatellite exploration in the climbing hydrangea (Hydrangea petiolaris Siebold & Zucc.) transcriptome: A resource for population genetics and functional genomics ## Description of the data and file structure There are two datasets for this article. Dataset 1 (filename: c320hanIDssrReads.fa) is a fasta file of 33,556 transcripts, which contain microsatellites, from Hydrangea petiolaris (Hydrangeaceae). \# The transcripts were generated using Trinity v2.15.1 program using RNA-seq raw reads. \# Final transcripts were filtered by TransRate v1.0.3 and RSEM v1.2.15 programs. \# The final transcript were utilized to identify microsatellite loci using the MIcroSAtellite identification tool (MISA). Dataset 2 (filename: c320hanID.p2top6AA.fa) is a fasta file of 27,006 ORFs from the 33,556 transcripts. \# These best ORF generated by TransDecoder v.5.5.0 programs.
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2025-07-26
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