Investigation of the role of N-terminal region in G protein-coupled oestrogen receptor 1 (GPER) localisation

This dataset comprises the complete experimental records, raw data files, and analytical outputs generated for the master's dissertation entitled “Molecular determinants of GPER subcellular localisation and the protective association of the P16L variant in a South African cohort." The dataset includes molecular cloning and construct validation data documenting the generation of HA-tagged human, murine, and human–murine chimeric G protein-coupled oestrogen receptor (GPER) expression constructs using restriction enzyme-based cloning and mutagenesis strategies. Supporting materials include plasmid maps, primer design records, restriction digestion validation sheets, ligation screening data, colony PCR confirmation results, and Sanger sequencing chromatograms verifying construct fidelity. The dataset further contains Chemidoc-generated agarose gel electrophoresis files, including original scan files and exported image formats, documenting restriction digests, colony PCR screening, and cloning validation. These files represent the original, unedited primary data confirming successful construct generation.The dataset also includes raw confocal microscopy image files obtained from transiently transfected HEK293T cells expressing wild-type and chimeric GPER constructs. These files include individual fluorescence channel images, merged composite images, imaging acquisition parameters, and experimental condition documentation. These data form the primary evidence used to assess molecular determinants governing GPER subcellular localisation. In addition, the dataset contains Restriction Fragment Length Polymorphism (RFLP) genotyping data generated to determine the prevalence and potential protective association of the GPER P16L variant within a South African cohort. Genomic DNA was extracted from clinical samples, amplified via PCR, and subjected to restriction enzyme digestion to distinguish allelic variants. The dataset includes patient sample inventory records, raw agarose gel electrophoresis images (including Chemidoc files), genotype interpretation records, and Excel-based tracking spreadsheets linking sample identifiers to corresponding digestion patterns and genotype calls. Collectively, this dataset constitutes the complete primary experimental evidence underpinning construct generation, receptor localisation analysis, and genetic association findings presented in the dissertation.