Identification of the IFN-stimulated genes in IFN-α2b-treated intrahepatic cholangiocarcinoma cells

Interferon-α2b (IFN-α2b) is a highly active cytokine protein that belongs to the interferon-α (IFN-α) family. As a pleiotropic cytokine, IFN-α2b has beneficial antiviral, antitumour, antiparasitic, and immunomodulatory activities. In vivo and in vitro, IFN-α2b can activate the JAK-STAT pathway (also known as IFN-I pathway) and function by inducing the transcription of downstream IFN-stimulated genes (ISGs).To identify the ISGs in intrahepatic cholangiocarcinoma (ICCA) cells and evaluate the therapeutic value of IFN-α2b, ICCA cells were treated with recombinant IFN-α2b protein. Total RNA samples were extracted and used for RNA-seq. Certain ISGs (e.g., MXs, IFITs, OASs) were found to be significantly upregulated upon IFN-α2b treatment. This dataset included two groups of samples. Every group contained three repetitions (rep). IFN-α2b was dissolved in the double distilled water with 1% fetal bovine serum (FBS), which was recommended by the suppliers. HUCCT1 cells were treated by IFN-α2b (60 ng/mL) for 24h. Another group of HUCCT1 cells underwent the same treatment with the recommended solvent. The total RNA was extracted and used for RNA-seq.