CASB: A concanavalin A-based sample barcoding strategy for single-cell sequencing [scRNA-seq]. CASB: A concanavalin A-based sample barcoding strategy for single-cell sequencing [scRNA-seq]

We reported a Concanavalin A-based Barcoding Strategy (CASB) for single-cell and single-nucleus sample multiplexing, which could be followed by different single-cell sequencing techniques. The method involves minimal sample processing, thereby preserving intact transcriptomic or epigenomic patterns. Besides sample multiplexing, the CASB could further improve data quality through doublet identification. Overall design: scRNA-seq of a mixture of 16 celltypes from 7 species