Representative raw data for the pressure-flow (P-Q) relationships in isolated perfused mouse lungs at different experimental conditions.

Representative pulmonary artery pressures (PAP; in mmHg) as recorded in individual isolated mouse lungs perfused at flow rates (Q) of 25, 50, 75, and 100 ml·kg−1·min−1 and corresponding intrinsic pulmonary vascular resistance values (R0; in mmHg·ml−1·kg−1·min−1) as calculated from the respective 4-point P-Q curve. Examples are given for normoxic (21% O2) or hypoxic (1% O2) lungs perfused with buffer alone (control), with RO 25-1553 at final concentrations of 0.01, 0.1 or 1 mg·mL−1, or with sildenafil (100 nMol·L−1). FiO2; fraction of inspiratory O2 (0.21 for normoxia; 0.01 for hypoxia).