Numerical data for densitometric quantification.
收藏Figshare2023-04-20 更新2026-04-28 收录
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Numerical data obtained from densitometric quantification of gel and western blot images using ImageJ version 1.53Q and calculations of averages and errors shown in Figs 1I and 2I. For Fig 1I, band intensities relative to background of the Map1 band in the Coomassie-stained gel were determined and normalized to the control with untreated RNCs. For Fig 2I, band intensities over background for bands corresponding to Naa25 and uL29 were determined for supernatant and pellet fraction, and the ratio of the Naa25 band intensities was calculated for each pair of fractions. Ratios were normalized by the band intensity measured for the ribosomal protein in each corresponding pellet fraction. Averages of these ratios were determined from the replicates and normalized to the wild type control experiment, where the binding efficiency in the control was set to 100%. Errors were calculated as standard deviations of the averages determined from replicates. (XLSX)
创建时间:
2023-04-20



