Additional file 4 of Exploring the lncRNA localization landscape within the retinal pigment epithelium under normal and stress conditions

Additional file 4: Supplementary Figure 4. Analysis of ADAR1 expression and localization. (A) Detection of ADAR1 by western blotting of lysates from ARPE-19 cells that were treated with H2O2 or left untreated. Blot images are cropped for clarity and conciseness. (B) Immunofluorescent staining of ADAR1 in ARPE-19 cells that were treated with H2O2 or left untreated. (C) Immunofluorescent detection of ADAR1-p110 in ARPE-19 cells that were treated with H2O2 or left untreated and that were transfected with either only ZsGreen mRNA (Neg. Control) or ZsGreen mRNA plus ADAR1-p110-Flag mRNA (ADAR1-p110). Successful transfection was visualized by fluorescence of the ZsGreen protein, and ADAR1-p110 expression was visualized via an anti-flag antibody.