DNA methylation changes in undifferentiated and differentiated human primary epithelial cells of antrum, corpus, and fundus

DNA methylation changes specific for healthy undifferentiated and differentiated human gastric primary epithelial cells of different regions of the stomach were here determined in order to describe the epigenetic profile of these cells in correlation to their gene expression pattern. Primary gastric epithelial cells were isolated from gastric sleeve resection material and cultivated as musosoids for 1-2 passages. DNA was isolated from the filter using the QIAamp DNA mini kit and DNA methylation patterns were determined using the Illumina Infinium EPIC methylation beadchip. Three biological replicates of six different conditions (undifferentiated and differentiated antrum, corpus, and fundus samples) were analyzed.