Effect of crpPg gene deletion on Porphyromonas gingivalis gene expression

Porphyromonas gingivalis is one of the main etiological agents responsible for initiating and progressing periodontal diseases in humans. This bacterium inhabits unfavorable conditions, therefore, to adapt to the changing external environment, e.g. redox conditions, and nutrient limitations, it must respond quickly to these stress factors. For this purpose, it uses transcription regulators. One of them can be the CrpPg protein classified to the CRP/FNR protein superfamily. The goal of this study was to investigate the properties of the CrpPg protein and identify the regulatory pathways it influences related to P. gingivalis virulence. For this, we generated ΔcrpPg mutant strain (A7436ΔcrpPg ) in the A7436 wild-type. Using RNA sequencing (RNA-seq), we examined how the deletion of crpPg affects gene expression in P. gingivalis. Raw data files for the A7436ΔcrpPg mutant strain are provided here, while those for the reference wild-type A7436 strain are available in the Gene Expression Omnibus (GEO; NCBI) under accession number GSE275439.