Gene expression profiling of renal tissues isolated from C57BL/6J mice treated with DMXAA

Flavonoid derivative DMXAA, also known as vadimezan or ASA404, is considered one of the most promising vascular disrupting agents that displays remarkable antitumor properties. To investigate the effect of DMXAA on gene expression in renal tissues, C57BL/6J mice were intraperitoneal injected with DMXAA or vehicle (10 mg/kg daily) for three days, then renal tissues were harvested and performed RNA-seq. C57BL/6J mice were treated with DMXAA (2 replicates) or vehicle (3 replicates) as described in the previous section. Mice received vehicle injection served as controls. Renal tissues were harvested to perform RNA-seq, and the data obtained from RNA-seq was used to perform comparative gene expression analysis.