RNA sequencing of an optimized shuttle cell line for intranasal delivery

We generated cell lines from LX-2 hepatic stellate cells that are otpimzed for intranasal delivery of the oncolytic adenovirus XVir-N-31 to treat glioblastoma. One cell line migrates faster, the next in addition expressed HSV-TK as a safety gene to eliminate cells in case of adverse events. RNA sequencing has been performed to analyze changes in gene expression between the parental (PAR) and the two optimized cell lines (FR and FR/TK). RNA sequencing was performed on mRNA generated from the three cell lines (n=3)