RNAseq of SMAD2/3DKO and Smad4KO in pluripotent mESC and EBs. RNAseq of SMAD2/3DKO and Smad4KO in pluripotent mESC and EBs

To dissected the roles of SMAD2/3 and SMAD4 in mouse embryonic stem cells (mESC), knockouts of SMAD2/3 (SMAD2 and SMAD3 double knockout, S2/3DKO) and SMAD4 (SMAD4 knockout, S4KO) were used to conduct RNAseq in ES and EB conditions. Overall design: WT, S2/3DKO and S4KO cells were collected at ES (D0) and embryoid body (EB) Day 4 conditions. Each sample were duplicated for RNAseq analysis.