Additional file 5: Table S1. of The intracellular plasma membrane-connected compartment in the assembly of HIV-1 in human macrophages

Quantitation of virus buds and membrane lengths for cryosections of monocyte-derived macrophages (MDMs) infected with budding-arrested HIV mutants. MDMs from four different donors were infected with the HIV-1 R3A mutants PTAP− or PTAP−YP−, and infected MDMs located by on-grid immunolabelling. Electron microscopy images were recorded and analysed by counting the number of virus buds seen either at the cell surface or within intracellular plasma membrane-connected compartments (IPMCs). Lengths of surface or IPMC membrane within the sections were measured by tracing (see Methods). Data from all of the cells summarised in Table 1. (XLSX 46 kb)