Levels of the endocannabinoids anandamide (AEA, pmol/g) in different brain structures during acute (16 h) and protracted (34 h) nicotine withdrawal phases.

Results showed that major changes occurred for AEA particularly in the amygdala, the hypothalamus and the hippocampus. Values represent the mean (±SEM) of subjects previously treated with nicotine compared to controls at respective time points: *p<0.05.#p<0.05, ##p<0.01, difference from control group at 16 h time point; +p<0.05, difference from nicotine group at 16 h time point.