DNA-binding assay by DBD-Threader on C2H2/C2HC zinc finger domains involved in protein-protein interactions.

Proteins were taken from tables 2 and 3 of reference [60]. We excluded two sequences from mouse, one (the ortholog of ZNF423/OAZ) from rat, and a long sequence (MBP-1, 2,700 AAs) not assessed by DBD-Threader. Protein EEA1 was not included because it has only one zinc finger domain and it was not detected by threading. We also discarded zinc finger domains known DNA-binding, e.g., domains from Sp1, YY1, Zac1, and BCL6. The vast majority of these DNA-binding domains, if not all, are positives. Plus and minus signs denote the positive and negative DNA-binding assessment on individual domains by DBD-Threader.
§ZF4 was mistakenly labeled as ZF3 in table 2 of reference [60].