Western blot
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下载链接：
https://figshare.com/articles/dataset/Western_blot/6497750
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资源简介：
Proteins
(30μg) extracted from cells were loaded and separated in 8% SDS-PAGE and then
transferred on nitrocellulose membranes. The membranes were blocked with 5%
non-fat milk and incubated with Anti-SP1 (1:1000, 9389, Cell Signaling
Technology, MA, USA) or Anti-CCR7 (1:1000, ab32527, Abcam, Cambridge, UK) in 5%
milk/PBS buffer at 4℃ overnight, followed by
incubation with goat anti-rabbit secondary antibody (A23720, Abbkine, Wuhan,
China) conjugated with DyLight fluorescent 680. After extensive wash with PBST
buffer, the band of protein was visualized under Odyssey CLx imaging system
(LI-COR, Inc., NE, USA).
创建时间：
2019-02-08
