Subcellular localization analysis of long non-non coding RNAs (lncRNAs) in skeletal muscle myofibers

In order to study the subcellular localization of lncRNAs in myofibers of skeletal muscle, single myofibers were isolated from Soleus, Extensor Digitorum Longus (EDL) and Tibialis Anterior (TA) of 9 mice. RNA was extracted from nuclei and cytoplasmic fractions of 5-10 isolated myofibers and than separately hybridized on microarrays. Preferential expression in nuclear or cytoplasmic compartment of each lncRNA was determined. Subcellular localization experiments were replicated using different pools of fibers from the same muscle of nine wild-type CD1 mice. Fibers were extracted from Soleus, EDL and TA for a total of 18 samples.