Clonal analysis.
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Single limbal epithelial cells were isolated from 6 primary co-cultures originated from crypt-rich and non-crypt rich limbal biopsies of three human donors. After 7 days, single clones were isolated and transferred to a new culture dish and expanded for 12 days prior to fixation and rhodamine staining. Clones were finally classified as holoclones, meroclones or paraclones depending on the percentage of aborted colonies. *is considered as statistically significant (Fisher’s exact test p
创建时间:
2015-12-02



