In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions [scRNA-seq]

The role of nutrient signaling processes in the fate decision of CD8 T cells are incompletely understood. By performing in vivo pooled CRISPR-Cas9 screening, we uncovered nutrient signaling processes underpinning the dynamics and heterogeneity of CD8 T cell fate decisions. Overall design: We used scRNA-seq to compare transcriptional heterogeneity of sgNTC ('spike for sgPofut1')- or sgPofut1-transduced P14 cells from the dual-color transfer system at day 7 post-infection from spleen; We used scRNA-seq to compare transcriptional heterogeneity of empty vector ('spike for NICD')- or NICD-expressing vector- transduced P14 cells from the dual-color transfer system at day 7 post-infection from spleen.