This project contains 12 samples (3 from mCAT HeLa, 3 from CHD1KO mCAT HeLa, 3 from CHD1KO plus CHD1WT and 3 from CHD1KO plus N- and C-terminal intrinsically disordered region deleted CHD1) of RNA-seq
Targeted DMS-MaPseq was applied to capture the intronic regions of the MYC pre-mRNA. DMS was used to modify RNA to generate mutations in cDNA sequence generated through mutational readthrough. Using t