RNA immunoprecipitation sequencing (RIP-seq) of human Dicer-bound miRNAs in HIV-1 Gag replicating cells.

Micro (mi)RNAs are critical regulators of gene expression in human cells, whose functions can be affected during viral replication. We showed that the Human Immunodeficiency Virus type 1 (HIV-1) Gag protein interacts with the human miRNA processor Dicer and modifies its retention of specific miRNAs without affecting the enzyme’s pre-miRNA processing activity. This sequencing experiment was performed to elucidate whether the presence of Gag in human cells has an effect on the distribution of miRNAs loaded onto Dicer. Profile of short RNAs immunoprecipitated with Dicer in cells expressing or not expressing HIV-1 Gag