Short chain fatty acid-mediated epigenetic modulation of inflammatory T cells in vitro

The present study examined the modulation of chromatin accessibility via ATACseq of CD4 T cells expanded in Th17 inducing conditions in the presence of the short chain fatty acid butyrate. Four groups were tested, Th17 conditions alone, and Th17 conditions with one of the following: 0.5 mM butyrate, 1 mM butyrate, or liposomal encapsulated butyrate (BLIPs). The goal was to enhance butyrate delivery via liposomal encapsulation, and demonstrate that BLIP treatment resulted in the same chromatin modulation as unencapsulated butyrate in CD4 T cells.