miRNA expression profiling of kidney collecting duct from mice lacking Dicer1 in AQP2 expressing cells.. miRNA expression profiling of kidney collecting duct from mice lacking Dicer1 in AQP2 expressing cells.

To investigate the role of miRNAs in the principal cell of kidney collecting duct, a Cre-LoxP recombination strategy was used to generate a mouse model lacking the endoribonuclease Dicer in AQP2 expressing cells (DicerAQP2Cre+). To identify miRNAs altered in DicerAQP2Cre+ mice, small RNA-sequencing analysis was performed on inner medulla tissue from 1-month-old DicerAQP2Cre+ mice and controls (n=3+3 mice). Small RNA-sequencing was used as, relative to probe-based microarrays or RT-qPCR plate arrays, this approach allows both the identification of miRNAs when they have been differentially processed and it offers the possibility to identify novel miRNAs. Overall design: miRNA expression profiles of 1-month-old DicerAQP2Cre+ mice and controls (n=3+3 mice).