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Detection of Legionella pneumophila Using a Real-Time PCR Hybridization Assay

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PubMed Central2026-05-16 收录
下载链接:
https://pmc.ncbi.nlm.nih.gov/articles/PMC87566/
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资源简介:
A real-time PCR hybridization assay for Legionella pneumophila is described; the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water sample. The assay, including DNA extraction and confirmation of product identity, is completed within 90 min of receipt of a sample.
提供机构:
American Society for Microbiology (ASM)
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