The P-body protein 4E-T regulates the balance between cell genesis and establishment of the postnatal neural stem cell pool by repressing mRNA translation [4ET_VSVZ]

To investigate the targets of 4E-T in the postnatal V-SVZ We performed RNA immunoprecipitations (RIPseq) on V-SVZ lysates anti-4E-T or control anti-IgG antibodies, isolated and sequenced the resulting RNA The SVZ was collected and lysed from P7/P8 CD1 animals, and immunoprecipitations were performed using anti-4E-T or anti-IgG control antibodies, and the associated mRNA was isolated