TMT-based quantitative proteomics strategy reveals the therapeutic mechanism of Banxia Xiexin decoction on a rat model of ethanol-induced chronic gastritis

In this study, using ten-plex tandem tag (TMT) labeling and mass spectrometry (MS)-based proteomics, 4,737 proteins in BXD were identified and quantified in the gastric antrum of rats. Compared with ethanol-induced gastritis group, the BXD group showed 258 differentially expressed proteins Bioinformatic analysis demonstrated that the identified BXD proteins regulated the PI3K/Akt, P53, and cell cycle signaling pathways. Further functional studies showed that 14-3-3 theta, Tenascin-C, and CASP3 played a crucial role in the treatment of ethanol-induced gastritis.