RNA sequencing of HCT116 and HKE3 colorectal cancer cell lines before and after stimulation with TGF-alpha

RNA sequencing was performed on HCT116 and HKE3 colorectal cancer cell lines before stimulation and at 15, 30, 60, 90 and 120 mins post-stimulation with transforming growth factor alpha (TGF-α) (0.01 µg/mL, Abcam). Three replicates were sequenced at each time point. RNAseq profiles from HCT116 and HKE3 cells before and after stimulation with TGF-alpha. In total, 36 cDNA libraries (2 cell lines X 6 time-points X 3 replicates per timepoint) were generated for sequencing.