Transformation efficiency increased in the N40 <i>bbe02::Bbluc-aadA</i> strain, compared to the wild-type strain N40 D10/E9.
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Plasmid pBSV2G DNA is used to transform B. burgdorferi strains by electroporation. Transformation frequency is calculated by the number of gentamicin-resistant colonies per 5x108 spirochetes, from a total of two experiments. Transformation efficiency increased in the N40 bbe02::Bbluc-aadA strain, compared to the wild-type strain N40 D10/E9.
创建时间:
2015-12-03



