Liver RNA-seq from obese mice following Roux-en-Y gastric bypass or sham surgery under chow or high-fat diet conditions

This study investigates how Roux-en-Y gastric bypass (RYGB) and high-fat diet (HFD) influence liver gene expression in mice. RNA sequencing was performed on liver samples from diet-induced obese male C57BL/6 mice that underwent either RYGB or sham surgery, followed by an 8-week post-operative period on either chow or HFD. Raw sequencing reads (FASTQ) and gene-level count data are provided to support investigation of RYGB- and diet-associated transcriptional changes in hepatic tissue. Overall design: Male C57BL/6 mice were fed a high-fat diet for 12 weeks to induce obesity, then underwent either Roux-en-Y gastric bypass (RYGB) or Sham surgery. Post-operatively, mice were maintained on either chow or high-fat diet for 8 weeks. The study included four groups: Sham Chow, RYGB Chow, Sham HFD, and RYGB HFD, with biological replicates in each group.