5hmC modification of monocytes and macrophages

RNA modifications are essential for the establishment of cellular identity. Although increasing evidence indicates that RNA modifications control the innate immune response, their role in monocyte-to-macrophage differentiation and polarisation is unclear. We profile 5hmC epitranscriptomes of monocytes and macrophages at resting, pro- and anti-inflammatory states. Overall design: 5hmC-IP-Seq was performed as described by Delatte, et al., and Lan et al.,.