Transgene integration typically takes place in an easy-to-transform laboratory variety before the transformation event is introgressed through backcrosses to elite cultivars. As new traits are added t
Study aimed to achieve high levels of multiallelic targeted mutagenesis in sugarcane using CRISPR/Cas9 to target the chlorophyll synthesis gene magnesium chelatase.
We find that pre-crRNAs comprising a full-length direct repeat (full-DR-crRNA) sequence with specific stem-loop G-C base substitutions exhibit increased editing efficiencies compared with the standard