Coculture with lin-/c-kit+ Stem Cells leads to a Paracrine Mediated Inhibition of Apoptosis in Cardiomyocytes

Conclusion: Our results provide evidence that coculture of SC with NRVCM leads to an upregulation of antiapoptotic genes and a paracrine-mediated increase in the phosphorylation of AKT. This results in less apoptotis in cocultured NRVCM. We therefore conclude, that cell-cell interactions lead to a modified gene expression in SC that may in part explain the cardioprotective effects of stem cell therapy.结论：本研究结果提供了确凿证据，表明干细胞（SC）与新生大鼠心室心肌细胞（Neonatal Rat Ventricular Cardiomyocytes，NRVCM）共培养可上调抗凋亡基因的表达，并通过旁分泌途径增强蛋白激酶B（AKT）的磷酸化水平，进而降低共培养体系中NRVCM的细胞凋亡率。据此我们认为，细胞间相互作用可改变SC的基因表达模式，这在一定程度上可解释干细胞疗法的心脏保护作用。