Micro-engineered perfusable 3D-bioprinted glioblastoma model for in-vivo mimicry of tumor microenvironment

We aimed to evaluated the effect of the cells growth conditions on the genetic profile of the cells. More specifically, we wanted to evaluate whether our 3D cancer model better reflects the in vivo scenario than 2D culture methods . Therfore, we performed RNA-seq of glioblastoma tumors grown in mice and compared it to a "penta-culture" comprised of GL261 murine GB cells, primary astrocytes, microglia, pericytes, and endothelial cells, grown in 2D or in 3D-bioink Overall design: Comparisom of gene expression between GL261 glioblastoma cells grown in mice to GL261 cells grown togather with the brain microenvironment cells (murine primary astrocytes, microglia, pericytes and endothelial cells) in 2D culture or in fibrin 3D-bioink. Bulk RNA.