Progesterone triggers Rho kinase – cofilin axis during in vitro and in vivo endometrial decidualization

Modified versions of the Supplementary Tables S II - S V and high-resolution versions of the Supplementary Figures S4, S5, S7 associated with the data that has been formally published in the following Human Reproduction article: https://doi.org/10.1093/humrep/deab161<br><b>Table S II</b>. Full list of proteomic data indicating label-free quantification intensities for proteins identified in at least two of the three samples of the same type. Abbreviations: NA, protein could not be detected in this sample type; PE, proliferative; SE, secretory.<br><b>Table S III</b>. Short-list of proteins (RMSD &lt;50% between 3 experiments) indicating abundances in proliferative or secretory ESCs. Abbreviations: NA, protein could not be detected in this sample type; PE, proliferative; SE, secretory.<br><br><b>Table S IV</b>. Full list of phosphoproteomic data indicating normalized intensities for phosphopeptides identified in at least two of the three samples of the same type. <br>Abbreviations: NA, phosphosite could not be detected in this sample type; PE, proliferative; SE, secretory.<br><b>Table S V</b>. Short-list of phosphosites (RMSD &lt;100% between 3 experiments) indicating abundances in proliferative or secretory ESCs. Abbreviations: NA, phosphosite could not be detected in this sample type; PE, proliferative; SE, secretory. Protein log₂FC values (based on proteomic data from Supplementary Table S II) are only shown for proteins bearing the phosphosites with significantly higher abundance in proliferative (log₂FC value &lt; -1) or secretory (log₂FC value &gt; 1) phase ESCs. Protein log₂FC below -5 or above 5 indicates proteins that could not be detected in secretory or proliferative ESCs, respectively. Targeting protein kinases are only shown for those phosphosites where the abundance of the corresponding protein (protein log₂FC value) could also be quantified based on the proteomic data.<br><br><b>Figure S4</b>. Functional enrichment analysis (based on Gene Ontology database) for proteins corresponding to phosphosites with significantly higher abundance (binary logarithm of fold change &lt; -1) in proliferative phase endometrial stromal cells.Abbreviations: BP, biological processes; CC, cellular components; GO, Gene Ontology; MF, molecular functions. g:Profiler web server (https://biit.cs.ut.ee/gprofiler/gost) was used for the analysis. The list of analysed proteins was based on the Supplementary Table S V. Only significantly enriched terms (g:SCS threshold value p_adj &lt; 0.05) are shown.<br><b>Figure S5</b>. Functional enrichment analysis (based on Gene Ontology database) for proteins corresponding to phosphosites with significantly higher abundance (binary logarithm of fold change &gt; 1) in secretory phase endometrial stromal cells.Abbreviations: BP, biological processes; CC, cellular components; GO, Gene Ontology; MF, molecular functions. g:Profiler web server (https://biit.cs.ut.ee/gprofiler/gost) was used for the analysis. The list of analysed proteins was based on the Supplementary Table S V. Only significantly enriched terms (g:SCS threshold value p_adj &lt; 0.05) are shown.<br><b>Figure S7</b>. Morphological variations promoted by the elevated activity of ROCK Rho-dependent protein kinase axis in the in vitro decidualized endometrial stromal cells (ESCs). (A-F) Representative Super Resolution Radial Fluctuations SRRF reconstructed images of treated ESCs derived from control (Ctrl; 1 donor) and PCOS polycystic ovary syndrome patient (PCOS; 1 donor) following fixation and staining with antibody against pSer3 of cofilin-1 (red), DAPI nuclear stain (blue), and phalloidin-Alexa Fluor® 488 (green). Treatment is indicated above the images, health status on the left; the scale bar (top left corner) is 20 microm. Abbreviations: E2, beta-oestradiol; IBMX, 3-isobutyl-1-methylxanthine; P4, progesterone.