Membrane FKBP12–FRB binding dataset (Fluo-FRB titration, two-channel)

Experiment overview:Fluorescence images of FKBP12–TM–mApple cells were captured across a titration series of fluorescein-labeled FRB (Fluo-FRB) to quantify membrane binding via ratiometric two-channel analysis. Data organization: Folder structure: [condition].tif.frames/ (e.g., 0.0.tif.frames, 0.5.tif.frames) Naming rule: [condition]_C00[channel]Z00[Z-index].tif Conditions: Fluo-FRB titration (nM): 0.0, 0.5, 1.0, 2.0, 5.0, 10, 20, 30 Channels: C001 Fluo-FRB; C002 FKBP12–TM–mApple; C003 DIC Z-index: Z001–Z004 (top to bottom) Example: 0.5_C001Z001.tif (condition 0.5 nM, channel 1, Z-stack 1) Per-condition file count: 12 images (C001–C003 × Z001–Z004)