Caydasi2012 - Regulation of Tem1 by the GAP complex in Spindle Position Checkpoint - Ubiquitous inactive model

Regulation of Tem1 by the GAP complex in
Spindle Position Checkpoint - Ubiquitous inactive

This model is described in the article:

A dynamical model of the
spindle position checkpoint.

Caydasi AK, Lohel M, Grünert G,
Dittrich P, Pereira G, Ibrahim B.
Mol. Syst. Biol. 2012; 8: 582
Abstract:

The orientation of the mitotic spindle with respect to the
polarity axis is crucial for the accuracy of asymmetric cell
division. In budding yeast, a surveillance mechanism called the
spindle position checkpoint (SPOC) prevents exit from mitosis
when the mitotic spindle fails to align along the
mother-to-daughter polarity axis. SPOC arrest relies upon
inhibition of the GTPase Tem1 by the GTPase-activating protein
(GAP) complex Bfa1-Bub2. Importantly, reactions signaling
mitotic exit take place at yeast centrosomes (named spindle
pole bodies, SPBs) and the GAP complex also promotes SPB
localization of Tem1. Yet, whether the regulation of Tem1 by
Bfa1-Bub2 takes place only at the SPBs remains elusive. Here,
we present a quantitative analysis of Bfa1-Bub2 and Tem1
localization at the SPBs. Based on the measured SPB-bound
protein levels, we introduce a dynamical model of the SPOC that
describes the regulation of Bfa1 and Tem1. Our model suggests
that Bfa1 interacts with Tem1 in the cytoplasm as well as at
the SPBs to provide efficient Tem1 inhibition.

This model is hosted on
BioModels Database
and identified by:
BIOMD0000000702.
To cite BioModels Database, please use:
Chelliah V et al. BioModels: ten-year
anniversary. Nucl. Acids Res. 2015, 43(Database
issue):D542-8.

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