Quantitative Microplate Assay for Real-Time Nuclease Kinetics
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https://figshare.com/articles/dataset/Quantitative_Microplate_Assay_for_Real_Time_Nuclease_Kinetics/3193186
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资源简介：
Utilizing the phenomenon of nucleases exposing oligonucleotide phosphate backbones to phosphatases we present a novel quantitative method for kinetics of nuclease catalysis. Inorganic phosphate released from nuclease products by phosphatases could be quantified in real-time by a fluorescent sensor of inorganic phosphate. Two different nucleases were employed, showing the versatility of this assay for multiple turnover label-free nuclease studies.
创建时间：
2016-04-25
