Quantitative Microplate Assay for Real-Time Nuclease Kinetics
收藏NIAID Data Ecosystem2026-03-09 收录
数据链接：
官方服务：
资源简介：
Utilizing the phenomenon of nucleases exposing oligonucleotide phosphate backbones to phosphatases we present a novel quantitative method for kinetics of nuclease catalysis. Inorganic phosphate released from nuclease products by phosphatases could be quantified in real-time by a fluorescent sensor of inorganic phosphate. Two different nucleases were employed, showing the versatility of this assay for multiple turnover label-free nuclease studies.
创建时间：
2016-04-25
