Primers for RT-qPCR.

Primers used for real time-PCR. We checked the expression levels of glucagon, insulin, PEPCK. All three genes in HCD-HG group were expressed higher than those in the control group, displaying some characteristics of type 2 diabetes. In zebrafish, there are two insulin isoforms named Insa and Insb. Insa functions similarly to the mammalian homologue in glucose regulation, whereas the Insb plays a more important role in development. Therefore, in our study we take Insa as the index of total insulin.#Pck1: phosphoenolpyruvate carboxykinase (PEPCK).