Probing downstream effects of ALK and ROS1 fusion oncogenes in Glioma using proteomics

To identify targets of aberrantly expressed RTK-fusion oncogenes, serine/threonine and tyrosine phosphorylation signatures in transgenic cells lines expressing different RTK-fusion oncogenes were compared with their respective kinasedead mutant counterparts. Comparing the differentially phosphorylated proteins between the different RTK fusions were used to identify commonly deregulated pathways as well as pathways specifically targeted by individual RTK-fusions.