The Piezo1-ATF3-PPP1r15a axis transduces mechanical stress into apoptosis in glioma under low-intensity focused ultrasound

This study aims to evaluate the therapeutic effects of LIFU on GBM and investigate its mechanisms of action. Overall design: U251 cells were exposed to LIFU (1 W/cm2) for 40 s, followed by three bioduplications, and RNA was extracted using Trizol. Nuohe Zhiyuan (Tianjin, China) performed RNA sequencing and analysis. Then, it was subjected to Illumina sequencing.