Next generation sequencing transcriptome analysis of colonic epithelial cells from bone marrow transplant recipient mice

Alloimmune T cell mediated gastrointestinal graft-versus-host disease (GI-GVHD) occur after bone marrow translantation (BMT). GI-GVHD cause significant morbidity and mortality. Immunosuppression therapies have shown good results with adverse effects, but are still incomplete. The biology of GVHD are often understood from the immune cell perspectve, but the pathogenesis and severity from host IEC traget cell perspective remain undefined. We used next generation sequencing transcriptome to detail the gene expression of colonic epithelial cells underlying the profile changes between the syngeneic recipient and allogeneic recipient. Overall design: C57BL/B6 mice used as recipients. Donor mice were C57BL/B6 for syngeneic group and BALB/c for allogeneic group. Recipient mice were received 1000cGy TBI on day -1 and were injected 2 million CD90.2+ T cells and 5 million bone marrow cells on day 0. Colonic epithelial cells were isolated from recipient mice on BMT day7 and processed for RNA isolation. Naive C57BL/B6 mice mached with age and sex were seclected .